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Fig. 1. Developmental mRNA expression pattern of the zebrafish cdx1b
gene. cdx1b mRNA was detected in one-cell zygote (A),
cleavage (B), blastula (D), shield (E,F), 80%
epiboly (H-J), 1-2s (L,M), 18s (O-Q), 20 hpf
(R-T), 28 hpf (V), 36 hpf (W), 48 hpf (X), 72 hpf
(Y) and 96 hpf (Z) stages.
(C,G,K,N,U) Embryos hybridized with the
sense RNA probe. Double-labeled in situ hybridization showed the expression of
cdx1b compared with either rx1, myoD (O,P) or shh
(Q). The two lines in R show different boundaries of a 20 hpf embryo viewed at
higher magnification in S and T. (a) Cryostat transverse section along
the plane of the line shown in Z. (b) Semiquantitative RT-PCR revealed
cdx1b expression levels in embryos from different developmental
stages. a, anus; d, diencephalon; i, intestine; mb, midbrain; n, notochord;
pp, prechordal plate; r, retina; s, somite. Scale bars: 100 µm.
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