DEV014316 Supplementary Material

Files in this Data Supplement:

• Supplemental Figures S1-S11 (Adobe PDF) -

  Figs. S1-S11. 4D analysis of embryos. Table of schemes showing the 8-cell stage, the 32-cell stage before cavitation, and the blastocyst of all 66 embryos analysed. The centres of gravity of the regions of the 8-cell-stage descendants are shown for the 32-cell stage before cavitation and for the blastocyst (for colour code and details see Fig. 2A-D). Embryos are sorted by class. Whether the blastocyst is embryonic/abembryonic (green square), half-half (orange square) or mixed (red square) is indicated.

• Supplemental Table S1 -

  Adobe PDF

• Movie 1 -

  Movie 1. Time-lapse imaging of early mouse development. Movie showing a merge of fluorescence and DIC to illustrate our method. The nuclear staining using EGFP-tagged histone H2B (Hadjantonakis and Papaiannou, 2004) can be seen. To observe all details in the movies, please also play using the left and right cursors. The movies were made using Adobe Image Ready, Volocity (Improvision) and ImageJ.

• Movie 2 -

  Movie 2. 4D analysis of normal mouse development. DIC movie and the corresponding 3D representation of an embryo from the 2-cell stage to blastocyst. The 3D representation illustrates the movement of cells by mitoses and the rotation of the embryo.