DEV013805 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Apoptosis of photoreceptors at the periphery of the pupal retina. Apoptosis of photoreceptors at the edge of the wild-type pupal retina is present by 35 hours APF (A-C) and continues to 40 hours APF (D-F). Pupal retinas were stained with antibodies against activated caspase (green) and Elav (blue). Elav is expressed in all photoreceptors.

• Supplemental Figure S2 -

  Fig. S2. Loss of Pygopus reduces Hth expression in photoreceptors. Pupal retinas were stained with antibodies against Hth (green), Elav (blue), and β-gal (magenta). Pygopus null mutant mitotic clones are indicated by the loss of β-gal expression and show reduced Hth staining when compared with surrounding peripheral photoreceptors.

• Supplemental Figure S3 -

  Fig. S3. Expression of Apc1 and Apc2 in pupal retinas. (A) Pupal retinas were stained with antibodies against Apc1 (green) and Arm (red). Apc1 and Arm staining are both observed at the apical surface of photoreceptors, but do not co-localize. (B-D) Pupal retinas containing Apc2³³ mutant mitotic clones were stained with antibodies against Apc2 (green) and β-gal (magenta). Apc2 staining is reduced in Apc2³³ mutant clones, which are indicated by loss of β-gal expression, confirming the specificity of the Apc2 antiserum. Apc2 expression is observed in most, if not all, retinal cell types.

• Supplemental Figure S4 -

  Fig. S4. Genomic organization of Apc2 and adjacent genes. Deletions in the Apc2³³ and Apc2⁷⁹ alleles are diagrammed. Insertion sites of the two P elements, EY09246 and G5028, used to generate these deletions are shown in green. Apc2³³ retains a fragment of the EY09246 P element (red arrowhead).

• Supplemental Figure S5 -

  Fig. S5. Increasing the Apc2 gene dosage does not affect the width of the dorsal rim area. (A) Pupal retina from a fly expressing two copies of an Apc2 transgene, PApc2^13.5. Second and third row ommatidia expressing Hth (green) are marked with yellow arrowheads. Photoreceptors are marked with anti-Elav (blue). (B) Average number of second row ommatidia expressing Hth in wild-type and PApc2^13.5 retinas. Standard errors are shown.