|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Rump associates with nos mRNA in vivo. (A)
RT-PCR to detect nos in RNA co-immunoprecipitated from wild-type (WT)
and rump1 Drosophila ovaries with either anti-Rump or
anti-β-galactosidase antibody. Poly(A+) RNA serves as positive control
for RT-PCR. Reactions were performed with (+) and without (-) reverse
transcriptase and products were visualized with ethidium bromide. Molecular
weight standards in the first lane correspond to the 1 kb-Plus Ladder
(Invitrogen). Beneath is shown an immunoblot of protein from each IP sample
with anti-Rump antibody. (B) RT-PCR for nos (top) and
anti-Rump immunoblot (bottom) as in A. Samples from WT (W) and
rump1 (r) 0- to 2-hour embryos are indicated. Reactions
were carried out with (+) or without (-) reverse transcriptase (RT) using
total RNA (T) from the extracts used for IP as a positive control or RNA
co-immunoprecipitated with anti-Rump antibody.
|
