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Figure 1


Fig. 1. Dia localization during morphogenesis. Wild-type Drosophila embryos: anterior leftwards, antigens indicated. (A-C) Cellularization. (D,E) Gastrulation. (A-B',D-D''') Cross-sections. (C,E) Surface views. (E') Diagram of A-E. (F-F''') Epidermis at stage 8. Sqh-GFP and AJs enriched at A/P (yellow arrows) or D/V (blue arrows) cell borders. Dia expression is symmetrical. (G) Mitotic domains. Cytokinetic furrows (arrows); MidBody (arrowhead). (H) Stage 10. (I) Stage 12. (J-K''',M,N) Dorsal closure. (J,J') Leading-edge AJ (arrow) and cortex (arrowhead). (K-K''') Amnioserosa showing drop-out cells (arrows). (L) Immunoblot: wild-type or e22c-GAL4xEGFP::Dia embryonic extract, probed for Dia or tubulin control. (M-O2) e22c-GAL4xEGFP::Dia live. (M) Inset shows donut-shaped structures in amnioserosa cytoplasm. Similar puncta are seen with Dia antibody (J,L). (N) Leading-edge AJs (arrows). Cell cortex (arrowheads). (O,O2) Segmental groove cells. Dia D/V planar polarization (O1, arrows). (P) Quantitation of EGFP::Dia planar-polarity in Groove cells. Error bars indicate s.d. Scale bars: 20 µm.





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