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Figure 2


Fig. 2. DiaCA triggers precocious amnioserosal apical constriction. Drosophila embryos: anterior leftwards, genotypes and antigens indicated. (A) Dia and DiaCA. (B-H,J,K,M-Q) Dorsal closure. (B) DiaCA expression in entire amnioserosa (c381-GAL4) (inset, DE-cad). (C-D'') DiaCA expression in individual cells. Constricted cells expressing DiaCA (arrowheads) and stretched wild-type cells (arrows). (E,E') Wild-type amnioserosal cells have irregular shapes. (F-G'') DiaCA expression in entire amnioserosa (F) versus wild type (G). (H,H',J,J') DiaCA expression in large group of amnioserosal cells showing Arm (H) and Myosin (J) accumulation. (I) Apically constricting amnioserosal cells (surface view left, cross-section right). (K) Myosin in wild-type drop-out cell (arrow). (L) Immunoblot: 12-16 hour DiaCAxe22c-GAL4 and wild-type embryos probed for MHC and {alpha}-catenin. Peanut is used as a loading control. (M-Q) Movie frames showing Sqh::GFP under its own promoter. (M1-M3) Wild type. (N1-O5,Q,R) DiaCA expression in amnioserosa (AS; c381-GAL4). (N1-N3) Drop-out cells (arrowheads). (P,Q) Higher magnification views. (R) Cuticle: DiaCA expression in amnioserosa. Scale bars: 20 µm.





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