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Figure 3


Fig. 3. DiaCA induces planar-polarized cell shape changes in the epidermis. Drosophila embryos: anterior leftwards, genotypes and antigens indicated. (A-C) Stage 16. Deep segmental grooves (arrows). (D-M) Dorsal closure. (D) Wild-type groove cells (arrows). (E-F''') DiaCAxen-GAL4. DE-cad D/V enrichment and F-actin accumulation (arrows). (G) Myosin in elongating wild-type epidermis. (H-I'') Wild-type groove cells. Myosin, DE-cad and EGFP::Dia (e22c-GAL4) D/V enrichment (arrows). (J,J') DiaCA D/V enrichment. (K-M) DiaCAxprd-GAL4. (K'',K''') Region indicated by yellow bracket in K'. DE-Cad and Myosin D/V enrichment (arrows). (L) Movie frame showing Arm::GFP. (L,M) DiaCA-expressing cells delimited by double-headed arrows. (N,O) Quantitation of planar polarization in indicated cell types. Error bars indicate s.d. (P) Wild-type elongating and groove cells. Scale bars: 20 µm.





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