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Figure 4


Fig. 4. Activating Myosin partially mimics DiaCA. Drosophila embryos: anterior leftwards, genotypes, GAL4 drivers and antigens indicated. (A-D') Laterial view of stage 13 embryos of indicated genotypes. (E-H'') Dorsal view of stage 13 embryos of indicated genotypes. (A-H'',K,K') Dorsal closure. (I-J') Stage 16. (A,A',E-E'') Wild-type. (B,B',D,D') DiaCA or MLCKCA expression induces deep grooves (arrowheads). (F-F'',H-H'') DiaCA- or MLCKCA-expressing amnioserosal cells. (C,C') SRF-VP16 expression. Elevated Myosin without deeper grooves (arrowheads). (G-G'') SRF-VP16-expressing amnioserosal cells. (I) Wild-type; grooves are no longer present (arrowheads). (K,K') DiaCA expression in zip zygotic mutants still leads to amnioserosal cell rounding. (J-K') DiaCA expression in zip zygotic mutant. Persistent grooves (J,J', arrowheads). Cortical actin alterations are slightly reduced (see Fig. 3E). Scale bars: 20 µm.





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