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Figure 7


Fig. 7. Reducing Dia function destabilizes AJs. Drosophila embryos: anterior leftwards, genotypes and antigens indicated. (A,B) Wild type. (C,D,I) Zygotically-rescued dia5M. (E-H) dia5M/Z; note less continuous AJs. (I-K) DE-cad. (J-K) dia5M/Z. (I2,J2 insets) Cross-sections. (L) Immunoblot showing 6-8 hour wild-type and dia5M/Z embryo extracts. {alpha}-tubulin is used as a loading control. (M-N',P,P',R-S') dia5M/Z. (O,Q,Q') Wild-type. (P,P') Increased apical Rab5-positive vesicles; some colocalize with DE-cad (arrows, lines). (R,S) Abnormal cortical blebbing (arrows). Scale bars: 20 µm.





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