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Fig. 8. Reducing Dia function disrupts tissue borders and increases protrusiveness. Drosophila embryos: germband retraction, anterior leftwards, genotypes and antigens indicated. (A,C) Wild-type. (A) Entire germband. (C,C') Higher magnification of the tail. Note smooth amnioserosa/epidermal tissue boundary (arrows). (B,D-G) Abnormal amnioserosal protrusions (arrows). (B,D,E-E'') dia/Rho1. (F,F') dia⁵M/Z or dia⁵M. (G) Progeny of dia² +/+ RhoGEF2⁰⁴²⁹¹ mothers. (H1-H3) Movie frames showing Moesin::GFP-expressing dia/Rho1 embryos. Abnormal protrusions over other amnioserosal cells (blue arrows), lateral and head epidermis (red arrows), or caudal epidermis (yellow arrows). (I) Movie frame showing Moesin::GFP-expressing wild type. (J-K'') MLCK^CA expression rescues protrusiveness of dia/Rho1 (J,J', arrow). (L) Epidermal cell protrusions. Scale bars: 20 µm.

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