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Fig. 4. Hair shaft production is severely impaired in
Catnb
ex3K14/+ skin.
(A,B) Advancing hair development was examined by transplanting
back skin from E17 embryos to nude mice. After 5 weeks, dense hair was present
in control graft, whereas the Catnbex3K14/+ mutant
graft had produced only a few hairs. (C-F) Light microscopic
examination revealed no major differences in hair structure between control
and mutant grafts. Arrows indicate zig-zags and arrowheads indicate awl hairs
(C,E). High-magnification pictures (D,F) show internal structure of awl hairs.
(G-N) Histological analysis of control (G,I) and mutant (H-N) grafts
after 3 (G,H,K,L) or 5 weeks (I,J,M,N). Control skin showed regular row of
catagen (G) and anagen-stage hair follicles (I), but in the mutant graft only
few well-developed follicles were observed (arrows in H and J). In the mutant
skin grafts, the down-growth of hair follicles was dramatically impaired and a
variety of abnormalities were obvious (K-N): extra sebocytes in large clusters
(arrows in K and L), epithelial budding from the outer root sheath of aberrant
follicles (arrows in M) and large epithelial cysts filled with keratin-like
material (arrowheads in J,L,N). Scale bars: 2 mm in A; 200 µm in C,E,G,I;
100 µm in D,F,L-N; 50 µm in K.
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