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Files in this Data Supplement:
Movie 1. Long-term video microscopy followed by image processing illustrates directed movement trajectories of cardiac progenitor cells during development. After electroporation of HH3 primitive streak and grafting into unlabeled, stage-matched host embryos, bright-field and dark-field images were collected as described in the Materials and methods to reveal cardiac progenitor cell movements. The data was exported as TIFF or JPEG files and tracks of individual cells were generated by successive logical addition of images with software written with the Optimas VI imaging library. The initial frames are shown in yellow and the final frames, collected towards the end of imaging, are shown in green. The movie shows dark-field images only and corresponds to the still images shown in Fig. 2A. Anterior of the embryos is to the right and posterior to the left.
Movie 2. Long-term video microscopy followed by image processing reveals altered movement trajectories of cardiac progenitor cells in the presence of Wnt3a cell pellets. The movement behaviour of cardiac progenitor cells was challenged by placing a pellet of Wnt3a-expressing cells into the position indicated in Fig. 2I. The movie was generated as described in the legend for Movie 1. Anterior of the embryos is to the top right and posterior to the bottom left. Wider movement tracks were observed in the presence of Wnt3a-expressing cells or following the electroporation of a Wnt3a-expression plasmid into the primitive streak.
Movie 3. Wnt3a-expressing cells repelled cardiac progenitors on the area opaca. This activity was independent of Fgfr1. Long-term video microscopy of primitive streak explants was followed by image processing as described in the legend for Movie 1. The movie corresponds to the still images shown in Fig. 3D, where the position of Wnt3a-expressing cells is indicated by yellow asterisks. HH3 primitive streak cells electroporated with GFP (on the right) or with a dominant-negative form of the Fgf receptor 1, Δ-FGFR1-YFP (on the left), migrated away from Wnt3a-expressing cells, which were located in between the two explants.
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