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Fig. 3. Wnt3a-expressing cells repell cardiac progenitors on the area opaca.
(A) Illustration of GFP-positive primitive streak explants cultured in
the presence of a cell pellet on the area opaca of an HH4 host embryo.
(B) Merged bright-field and fluorescent images of a representative
streak explant and cell pellet on the area opaca. Still images from the start
(0h) and end points (16h) of the experiments are shown. (C) Image
tracks of movies showed that HH3 primitive streak cells migrated in all
directions away from a graft cultured on the area opaca and ignored the
presence of a control cell pellet (yellow asterisk). (D) HH3 primitive
streak cells electroporated with GFP (on the right of the cell pellet) or with
a dominant-negative form of Fgf receptor 1, 
-Fgfr1-YFP (on the left of
the cell pellet) migrated away from Wnt3a-expressing cells (see Movie 3 in the
supplementary material). (E) HH4 primitive streak cells did not respond
to Wnt3a-expressing cells. (F) HH3 cardiac progenitors were repelled by
a bead soaked in Fgf8. (G) HH3 cardiac progenitors electroporated with
DN-Wnt3a-GFP no longer responded to Wnt3a-expressing cells. (H)
Wnt11-expressing cells repelled HH3 prospective cardiac cells. Yellow
asterisks indicate the position of cell pellets or beads. 0h, start of
imaging; 16h, end of imaging; ao, area opaca; ps, primitive streak.
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