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Fig. 4. DN-Wnt3a can rescue the effects of Wnt3a on cell movement patterns in
vivo. (A) DN-Wnt3a-GFP-(green) and RFP-(red) expressing HH3 streak
cells grafted into a stage-matched host displayed normal migration patterns in
control embryos. (B) A graft of Wnt3a-expressing cells affected GFP-
and RFP-labelled cells at different anterior-posterior levels and caused cells
to take a wider path. (C) The movement trajectories of cells expressing
DN-Wnt3a-GFP were restored to normal in the presence of Wnt3a-expressing cells
but RFP-transfected cells still displayed wider migration trajectories. (A-C)
Panels on the left show GFP and RFP fluorescence with bright field at the
start point of imaging. The two central panels show dark-field images at the
time points indicated. Panels on the right show fluorescence with bright field
at the end of imaging. Vertical white lines indicate the axial midline; angled
white lines indicate the main trajectory of cells leaving the streak.
Heart-forming regions at HH8 [based on Stalsberg and DeHaan
(Stalsberg and DeHaan, 1969)]
are outlined in white. Scale bar in A: 
500 µm.
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