DEV013359 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 - Adobe PDF

• Supplemental Table S2 - Adobe PDF

• Supplemental Table S3 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Heparin inhibits Rspo2 cell surface binding. Untransfected HEK293T cells were absorbed with the indicated conditioned media in the presence of heparin and cell bound AP activity assayed. The binding of Rspo2AP and Δ212-243AP were effectively competed by increasing the amount of heparin in the media.

• Supplemental Figure S2 -

  Fig. S2. Immunohistochemical analysis of cell specific markers in E18.5 fetal lungs of the indicated genotypes. (A-D) FoxJ1 staining indicated that proximal airways in all samples possessed ciliated epithelial cells. (E-H) Pecam1 was normally expressed in the vascular endothelial cells of large vessels and capillaries within the distal mesenchyme. (I-L) Smooth muscle actin positive cells were observed in tissues of all genotypes surrounding proximal airways and dispersed throughout the distal mesenchyme. Scale bars: 10 µm in D,H,L.