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Figure 1


Fig. 1. Rspo2 is disrupted in the transgene insertional mutation footless. (A) Rspo2 gene. Untranslated exons are shown in black, arrows indicate the location of PCR primers. (B) Northern blot of E11.0 embryo total RNA. Arrow indicates full-length transcript; arrowhead indicates alternatively spliced transcript. (C) RT-PCR of E11.0 embryonic cDNA. The primers for exons 3-6 spanning the transgene integration site detected only small amounts of product in Rspo2Tg/Tg embryos.





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