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Figure 6


Fig. 6. Branching defects in Rspo2Tg/Tg lungs. Organ culture of E11.5 lungs in the presence of conditioned media containing either AP or Rspo2 {Delta}212-243AP. (A) Rspo2 {Delta}212-243AP partially rescued the branching defect observed in Rspo2Tg/Tg lung buds. (B) Counted epithelial tips of mutant lung buds in culture. (C,D) Rspo2+/+ and Rspo2+/Tg lung buds grew similarly in culture in response to either AP or Rspo2 {Delta}212-243AP. (D) Number of distal tips of individual lung buds observed within a representative experiment.





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