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Figure 7


Fig. 7. Rspo2Tg/Tg lungs exhibit reduced Wnt signaling. The Rspo2Tg allele was crossed into the Wnt signaling reporter TOPGAL line and β-galactosidase activity examined in whole-mount lung specimens at E11.5: Rspo2+/+ (A), Rspo2Tg/Tg (B,C,E). A, B and E are littermates. White arrows indicate high levels of Wnt signaling activity in the distal tips of wild-type lungs compared with the lower level in the distal tips of mutants (arrowheads). (D) Quantitation of β-galactosidase activity in E11.5 lung buds. (F) Semi-quantitative real-time RT-PCR of E11.5 cDNA samples, indicating that Irx3 is reduced threefold. (G,H) Sox9 is expressed in a normal proximal distal pattern in Rspo2+/+ (G) and Rspo2Tg/Tg (H) lungs.





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