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Figure 4


Fig. 4. Analyses of Runx1{Delta}4/{Delta}4 bulge SC numbers and gene expression. (A) Skin sections from wild-type and {Delta}4 mice at PD21 show expression of markers indicated at the top (yellow). Bu, bulge; hg, hair germ; DP, dermal papillae. Asterisk indicates background signal of hair shaft. (B) Surface expression of CD34 and {alpha}6-integrin by FACS of skin cells at PD20. (C) Summary of FACS experiments in B shows frequency of {Delta}4 and wild-type CD34+/{alpha}6-integrin+ bulge cells in the skin (P=0.2 demonstrates no significant differences). (D) Bulge (Bu) and outside the bulge (O/Bl) sorted cells from (B) were used to prepare total RNA and cDNA. RT-PCR analyses show expression levels for genes indicated on the left. +/+ and -/+ designate CD34 and {alpha}6-integrin expression in each population. The last four lanes are negative controls without reverse transcriptase. (E) Summary of phenotypes for mutant mice indicated (left column) and gene expression level obtained consistently in wild-type and {Delta}4 mice tested (right column). Tm, targeted mutation (knockout); Tg, transgenic (overexpression). Level of expression in Runx1{Delta}4/{Delta}4 bulge is indicated in the right-hand column by + (increase), - (decrease) or N/C (no change). N/A, not applicable.





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