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Figure 5


Fig. 5. Yolk protein trafficking is blocked in the unc-108(sm237) mutant. (Aa-d) Yolk protein uptake is normal in the developing oocytes of unc-108(sm237) mutant C. elegans. The accumulation of VIT-2::GFP in the developing ooctyes was examined in wild-type (bIs1) (a,b) and in the sm237 mutant [unc-108(sm237); bIs1] (c,d). (Ba-d) The yolk protein is redistributed to the intestine primordium (arrow) in the wild-type 1.5-fold (a,b) and 4-fold (c,d) stage embryos. (Ca-Dd) Yolk protein trafficking is blocked in the unc-108(sm237) mutant (C) or unc-108(RNAi) animal (D). 1.5-(a,b) and 4-fold (c,d) stage embryos were examined for the redistribution of VIT-2::GFP from the anterior region to the gut primordium. Abundant VIT-2::GFP signal could be observed in the anterior region of the embryos (arrow). Scale bars: 1 µm in A; 5 µm in B-D.





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