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Figure 7


Fig. 7. UNC-108 associates with phagosomes. (Aa,b) UNC-108::GFP clusters around cell corpses (arrow). DIC (a) and fluorescent confocal (b) images of a wild-type C. elegans embryo transgenic for Punc-108unc-108::gfp. (Ba-o) UNC-108::mCHERRY is recruited to the phagosome preceded by CED-1::GFP. DIC (a,d,g,j,m), confocal time-lapse images of CED-1::GFP (b,e,h,k,n) and UNC-108::mCHERRY (c,f,i,l,o) around the same cell corpse in a wild-type embryo. The time point was set as 0 minute when the CED-1::GFP ring was clearly seen. Images from five time points after that are shown. Arrows point to the cell corpse and to the corresponding fluorescent signals. (Ca-l) UNC-108::GFP co-localizes with mCHERRY::RAB-5, mCHERRY::RAB-7 and LMP-1::mCHERRY to the phagosome. DIC and fluorescent confocal images of a wild-type embryo transgenic for Punc-108unc-108::gfp and Pced-1mcherry::rab-5 (a-d) or Punc-108unc-108::gfp and Pced-1mcherry::rab-7 (e-h) or Punc-108unc-108::gfp and Pced-1lmp-1::mcherry (i-l). Arrows indicate the co-localization of UNC-108::GFP with mCHERRY::RAB-5, mCHERRY::RAB-7 or LMP-1::mCHERRY on the phagosome. Scale bars: 5 µm.





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