spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Auxilin is required only in the Delta signaling cells. (A) Structure of Drosophila Auxilin. The vertical black bars within the Clathrin-binding domain are DPF motifs that in GAK bind AP2 (Umeda et al., 2000; Korolchuk and Banting, 2002). Approximate sites of the mutations in three aux nonsense mutant alleles and one missense allele (K47) are indicated (Eun et al., 2007). (B,B') Confocal image of an aux727/auxD128 clone (outlined) marked by the absence of GFP. Clones were generated in larvae of the genotype w, eyFLP; FRT42D gaux+, Ubi-GFP/FRT42D; aux727/auxD128. (C) Diagram of three Notch/Delta signaling events near the morphogenetic furrow. I, proneural enhancement; II, lateral inhibition; III, R-cell restriction. Numbers at top indicate rows of developing ommatidia. Numbers inside circles indicate R-cells. Blue cells are ectopic R-cells when R-cell restriction fails. mf, morphogenetic furrow; a, anterior. (D-G') Confocal images of immunolabeled third instar larval eye discs. (D,D') aux727/auxD136 clone (outlined), marked by absence of GFP, generated in larvae of genotype w, eyFLP; FRT42D gaux+, Ubi-GFP/FRT42D; aux727/auxD136. R-cell nuclei are Elav+. Why Elav+ cells are observed that are not directly adjacent to the clone edge is not known. This was observed also in lqf- and Dl- clones (Baker and Yu, 1996; Overstreet et al., 2004). (E,E') aux727/auxD136 clone expressing β-galactosidase from a Delta enhancer trap (Dl-lacZ) generated in larvae of genotype w, eyFLP; Tub-aux+, Ubi-GFP, FRT40A/FRT40A; auxD136 Dl-lacZ/aux727. The arrow indicates furrow. (F,F') aux- clone generated as in B, outlined. The arrow indicates furrow. (G,G') aux- clone generated as in D, outlined. Arrows indicate three aux- E(spl)+ nuclei. There are few aux- E(spl)+ nuclei observed because most of the cells at the clone border are R-cells (Elav+, see D), which do not express E(spl). Also, additional E(spl)+ nuclei are observed in other planes. Scale bar: 20 µm in B,B',D-F'; 10 µm in G,G'.





Right arrow Return to article