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Figure 2


Fig. 2. Auxilin is required for some Delta internalization. Confocal images of immunolabeled third instar larval eye discs. Black-and-white images (A-D,E,F) show Delta protein. Arrowheads indicate furrow. (A) Wild type (wt). (B) Nts1 disc at 29°C for 6 hours. (C) lqf- clone (outlined). (D) lqf hypomorph heterozygous for strong neur mutation. (E-E'') auxK47/auxD136 showing Delta and F-actin (plasma membrane). (F-F'') aux727/auxD128 clone (outlined) marked by absence of GFP, generated in larvae of genotype w, eyFLP; FRT42D gaux+, Ubi-GFP/FRT42D; aux727/auxD128. (G) Same clone as in F, but more basal. Arrowheads indicate some of the numerous puncta. Scale bar: 10 µm in G for A-G.





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