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Fig. 7. Quantitation of Shh::GFP ligand puncta and basal bodies in early ventral
neural tubes. Eight-somite-stage mouse neural tubes cut at the level of
the heart were analyzed at three distinct volumes (V1, V2, V3) along the D-V
axis. (A) The relative positions of V1, V2 and V3 in the
xy-plane. Each volume is 9 µm wide (x-axis), 9 µm long
(y-axis) and 2.1 µm deep (a stack of six confocal images taken at
0.35 µm steps along the z-axis). Nuclei (blue), Shh::GFP ligand
(green), 
-tubulin (red). (B) Quantification of Shh::GFP ligand
puncta and basal bodies within each apical volume. A Shh::GFP punctum is
basal-body-associated if it is within 1 µm of a -tubulin punctum. A
nucleus is associated with an apical volume if it is laterally positioned to
that volume. Ventral midline nuclei are associated with V3. Note that the
percentage of Shh::GFP puncta associated with the basal body decreases
ventrally.
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