|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Anterior Osk, specifically long Osk, recruits endosomal proteins and
stimulates endocytosis in Drosophila. (A-D) Rbsn-5
localization at the oocyte anterior without (A) or with expression of
osk-bcd 3' UTR (B), osk(M1L)-bcd
3' UTR (C) or osk(M139L)-bcd
3' UTR (D). Rbsn-5 accumulated in the anterior of oocytes
expressing the intact osk-bcd 3' UTR or
osk(M139L)-bcd 3' UTR transgenes,
but not the osk(M1L)-bcd 3' UTR
transgene (arrowheads). (E-J) Rab5 (E,F), Rab11 (G,H) and Rab7 (I,J)
localization at the oocyte anterior without (E,G,I) or with long Osk
expression (F,H,J). Rab5, Rab11 and Rab7 all accumulated at the oocyte
anterior when long Osk was expressed (arrowheads). (K,L)
Incorporation of FM4-64 in stage 9 oocytes without (K) or with long Osk
expression (L). Ectopic dye incorporation was observed at the oocyte anterior
in the presence of long Osk (arrowheads). (M,N) Kin-βgal
localization without (M) or with long Osk expression (N). No ectopic
Kin-βgal signal was detected at the anterior pole of the
osk(M139L)-bcd 3' UTR oocyte
(arrowheads). DNA was counterstained with DAPI (cyan) in all images. Scale
bars: 20 µm.
|
