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Fig. 6. Rbsn-5 is required for Osk-mediated F-actin reorganization and the
anchoring of pole plasm components in Drosophila. (A-D)
Wild-type (A,B) or rbsn-5- (C,D) oocytes, without (A,C) or
with expression of osk-bcd 3' UTR (B,D), were stained
for Osk and F-actin. Enlargements of the bracketed regions are shown in the
right panels. Aberrant F-actin aggregates (magenta) that contained Osk (green)
were observed around the anterior region in the rbsn-5-
oocyte when the osk-bcd 3' UTR transgene was expressed
(arrowheads in D). (E-H) Tud protein localization in wild-type (E,F) or
rbsn-5- (G,H) oocytes without (E,G) or with expression of
osk-bcd 3' UTR (F,H). Arrowheads in H indicate
aberrant aggregates containing F-actin and Tud. (I-N) Rab5 (I,J), Rab11
(K,L) and Rab7 (M,N) localization to the oocyte anterior without (I,K,M) or
with expression of osk-bcd 3' UTR (J,L,N) in
rbsn-5- GLCs. Arrowheads point to the accumulation of
endosomal proteins in areas of ectopic Osk expression. DNA was counterstained
with DAPI (cyan). Scale bars: 20 µm.
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