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Fig. 5. Precision of the PMad gradient. (A) Immunostaining for PMad
(red). Same set of disks, orientation, source boundary and scale bar as in
Fig. 4A. (B) PMad FI in
individual nuclei as a function of the distance x from the Dpp
source, normalized to the total FI. The average PMad profile (red line) is
approximated by an exponential function
c-PMad(x)=P0exp(-x/
PMad)
with PMad=25.0 µm=9.6 cells. Inset, uncertainty
of the FI. (C)
Normalized PMad correlated to non-normalized GFP-Dpp level in the same disk
and for the same position. Inset, the same but normalized GFP-Dpp data.
(D) Relative concentration uncertainty
of PMad. Note the similarity to Fig.
4F. (E) Positional uncertainty
of the PMad gradient as
a function of the positional uncertainty of the non-normalized GFP-Dpp
gradient, 
. Inset, same
but with normalized GFP-Dpp data.
and
are proportional with a
proportionality constant (slope of the red line) close to
,
non-normalized GFP-Dpp data, and
,
normalized. Green horizontal bars in B,D, 5 cell diameters (13 µm).
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