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Fig. 5. Abrogation of hsp90a but not hsp90a2 function leads to upregulation of genes encoding proteins likely to be involved in sarcomere assembly. (A-I) Lateral views, at the stage indicated bottom left, showing expression of the genes indicated bottom right in wild-type (Sib) and mutant zebrafish embryos. Note that although hspa8l and unc45b are upregulated in the slo^tu44c allele, hsp90a itself is not. (J) Western blots using lysates of slo^u45 and slo^tu44c mutant and sibling embryos. More Hsp90 protein was present in slo^u45 mutant lysates than in the corresponding siblings. By contrast, slo^tu44c mutant lysates contained less Hsp90 protein than siblings. (K,L) Quantitative PCR (qPCR) analyses of levels of hsp90a and hsp90a2 expression in wild type (Sibs), slo^u45 and slo^tu44c mutants (Muts). Absolute expression levels of hsp90a (normalised to µg of total RNA) were significantly increased (K; P=0.002, n=5) in slo^u45 mutants compared with siblings; levels of expression of hsp90a in slo^tu44c mutants were unchanged compared with siblings (K; P=0.75, n=3); and levels of expression of hsp90a2 were not significantly different between slo^u45mutants and siblings nor between slo^tu44c mutants and siblings (L; P=0.813, n=5 and P=0.074, n=3, respectively).

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