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Fig. 4. Atrial progenitors are present in the posterior SHF on right and left
sides of Mlc3f-nlacZ-2E embryos at the 4-somite stage.
(A-H) Left (L) (A-D) and right (R) (E-H) explants of the pSHF from
Mlc3f-nlacZ-2E mouse embryos at the 4-somite stage, after 72 hours of
culture. (A,E) Hoechst staining; (B,F) myosin heavy chain (MHC)
immunofluorescence; (C,G) β-gal immunofluorescence. (D) Merge of B and C;
(H) merge of F and G. Scale bar: 10 µm in H for A-H. (I)
Quantitative analysis of β-gal and MHC double-positive cells, as a
percentage of the total number of cells, in 30 explants from each side of the
pSHF. White, percentage in left explants; black, percentage in right explants.
(J) qRT-PCR analysis on ten pooled explants from left (l) and right (r)
sides. lacZ (arrowheads) and Mlc2a transcript abundance is
expressed as a percentage of that of Gapdh transcripts.
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