DEV010694 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. The prostanoid synthesis and signaling pathway. Shown with the enzymes next to the arrows. Antagonists used are listed beside or under the protein in red, agonist in green.

• Supplemental Figure S2 -

  Fig. S2. Injection of tbxas MO leads to pericardial edema. At 72 hpf, larvae that had been injected with 2 ng of tbxas MO had pericardial edema (B), whereas uninjected controls did not (A).

• Movie 1 -

  Movie 1. 76 hpf larvae have a valve leaflet. SPIM movie of a Tg(flk1:EGFP)^s843 larva taken at 75 fps and shown at 30 fps.

• Movie 2 -

  Movie 2. 85 hpf valve leaflet. SPIM movie of a Tg(flk1:EGFP)^s843 larva taken at 70 fps and shown at 30 fps.

• Movie 3 -

  Movie 3. 63 hpf valve. SPIM movie of a Tg(flk1:EGFP)^s843; Tg(gata1:dsRed)^sd2 larva taken at 141 fps and shown at 30 fps.

• Movie 4 -

  Movie 4. 55 hpf valve. SPIM movie of a Tg(flk1:EGFP)^s843; Tg(gata1:dsRed)^sd2 larva taken at 114 fps and shown at 30 fps.

• Movie 5 -

  Movie 5. 72 hpf valve. SPIM movie of a Tg(flk1:EGFP)^s843; Tg(gata1:dsRed)^sd2 larva taken at 155 fps and shown at 30 fps.

• Movie 6 -

  Movie 6. 96 hpf valve. SPIM movie of a Tg(flk1:EGFP)^s843; Tg(gata1:dsRed)^sd2 larva taken at 147 fps and shown at 30 fps.

• Movie 7 -

  Movie 7. 102 hpf valve. SPIM movie of a Tg(flk1:EGFP)^s843; Tg(gata1:dsRed)^sd2 larva taken at 137 fps and shown at 30 fps.

• Movie 8 -

  Movie 8. Inhibition of ErbB signaling disrupts valve function. SPIM movie of a 72 hpf Tg(flk1:EGFP)^s843; Tg(gata1:dsRed)^sd2 larva treated with 4 µM AG1478 from 56-72 hpf taken at 141 fps and shown at 30 fps.

• Movie 9 -

  Movie 9. Inhibition of TGFβ signaling disrupts valve function. SPIM movie of a 72 hpf Tg(flk1:EGFP)^s843; Tg(gata1:dsRed)^sd2 larva treated with 3 µM SB431542 from 56-72 hpf taken at 122 fps and shown at 30 fps.

• Movie 10 -

  Movie 10. Inhibition of Cox2 signaling disrupts valve function. SPIM movie of a 72 hpf Tg(flk1:EGFP)^s843; Tg(gata1:dsRed)^sd2 larva treated with 25 µM CAY10404 from 56-72 hpf taken at 113 fps and shown at 30 fps.