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Fig. 3. Inhibiting ErbB signaling disrupted the rolling of the valve.
Zebrafish embryos were treated with 4 µM AG1478, an ErbB receptor
inhibitor, or 1% DMSO from 56-72 hpf and imaged with SPIM (A-C) or
stained with rhodamine-phalloidin and imaged with confocal microscopy
(D,E). Retrograde flow of blood cells per beat was quantified
for animals treated with 1% DMSO, 4 µM AG1478, 25 µM CAY10404 or 3 µM
SB431542 along with the 95% confidence interval (F). (A-C) Inhibition
of ErbB signaling led to altered valve function, with the valve coming
together on the ventricular side of the AVC (A), rolling ventricularly to
atrially (B), and coming apart during the relaxation phase allowing retrograde
blood flow (C,F). Larvae treated with inhibitor showed malformed valves with
incomplete invagination and increased space between the two sides of the valve
leaflet (E).
