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Fig. 4. Inhibition of TGFβ signaling disrupted the shape of
invaginating valve cells. (A-G) Zebrafish embryos were treated with
1% DMSO (A,C,E) or 3 µM SB431542 (B,D,F) from either 56-72 (A,B,E,F) or
56-96 (C,D) hpf, and stained with rhodamine-phalloidin (A-D) or for Alcam
(E,F) and imaged by confocal microscopy (A-F) or SPIM (G). The thickness of
the valve at the point of greatest compression or expansion was measured and
compared along with the 95% confidence interval (G). (A-D) Inhibition of
TGFβ signaling resulted in cuboidal invaginating cells (B,D) compared
with control valves (A,C). (E,F) TGFβ signaling was required for the
downregulation of Alcam in the invaginating cells. (G) This change in shape
and adhesion resulted in the inability of the valves to compress and
expand.
