(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
Fig. 5. Inhibition of Cox2 and prostaglandin signaling altered myocardial cell
shape. Zebrafish embryos were treated with 25 µM CAY10404
(A-C,E,G,M,N), a Cox2 inhibitor, or 1% DMSO
(D,F,K,L) from 56-72 (A-C,D-G) or 56-60 (K-N) hpf,
imaged using SPIM (A-C) or confocal microscopy (D-G,K-N) and projections made
(F,G,L,N). The area (I) and circularity (J) of cells in the
posterior outer curvature of the ventricle was measured and the 95% confidence
interval calculated. (A-C) Inhibition of Cox2 caused the invaginating cells to
be translated towards the ventricle, such that the AVC closed (A) and rolled
(B) on an endocardial monolayer leading to retrograde blood flow (B). (D,E)
The invaginating cells shifted towards the ventricle because the myocardium
overlying the superior AVC bent towards the ventricle (E). (F,G) This bending
correlates with changes in ventricular shape caused by decreases in cell area
(G,I) and increases in cell circularity (G,J). (H) The ventricle was
divided into four areas based on anterior or posterior, outer or inner
curvature, for the cell shape and circularity analysis. Significant
differences were found in the posterior outer curvature of the ventricle,
indicated with an asterisk. Myocardial bending (M) and cell shape changes (N)
arose within 4 hours of treatment with Cox2 inhibitor.
