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Fig. 6. Activation of TXA2 signaling or inhibition of PGF2
rescued
the Cox2 inhibition phenotype. Zebrafish embryos were treated with 10
µM SQ29548 (A,B), a TP receptor inhibitor, 25 µM CAY10404
and 5 µM AL8810 (H,I), an FP receptor inhibitor, 25 µM CAY10404 and 10
µM U46619 (J,K), a TP receptor agonist, or injected with tbxas
morpholinos (C,D), imaged with confocal microscopy (A-D,F-K),
projections made (B,D,G,I,K) and the area (L) and circularity (M) measured and
the 95% confidence interval calculated. Myocardial bending (A,C,F) and cell
shape changes (B,D,G) were observed in larvae in which TXA2 signaling was
inhibited (A-D) or PGF2 signaling was increased (F,G).
(E) RT-PCR was performed on RNA from isolated hearts for cox1,
cox2a, cox2b and tbxas. cox1,
cox2a, cox2b and tbxas are expressed in the heart.
(H-M) Inhibition of PGF2 signaling (H,I) or activation of TXA2
signaling (J,K) rescued the Cox2 inhibition phenotypes of myocardial bending
(H,J) and the decrease in myocardial cell area (I,K,L), but not the increase
in myocardial circularity (M).
