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Figure 1


Fig. 1. APP-binding sites in embryonic chick brain. (A,B) Binding of AP-APPs{alpha} versus AP control. Ventral view, E11.5-12.5 brains. Prominent binding in optic tectum (OT, arrow), with additional staining in olfactory bulbs (OB, arrowhead) and other regions. (C) AP-APPs{alpha} staining of tectum at greater magnification, showing anterior-posterior striation characteristic of axons in the stratum opticum. Anterior-posterior is horizontal. Scale bar: 100 µm. (D-F) Time-course of AP-APPs{alpha} binding to brains. Dorsal view. Anterior (A) and posterior (P) extremes of tecta. Arrows indicate posterior limit of staining. (G) Binding of AP-APPs{alpha} to brain from embryo with single enucleated eye. Ventral view. Staining in contralateral tectum (arrow) is reduced to background levels. AP, alkaline phosphatase.





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