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Fig. 2. Apoptosis occurs in differentiating neurons in the piy mutant
retina. (A-F) BrdU labeling (A,C,E) and TUNEL (B,D,F) of
piy mutant zebrafish retinas. At 32 hpf, many retinal cells
incorporate BrdU (A, red) and there are no TUNEL-positive cells (B, red). At
42 hpf, many neurons are produced in the central retina and these express the
neuronal marker ath5 (D, green). Apoptotic cells are observed in the
ath5-positive central retina, but are rare in the CMZ (D, red). At 72
hpf, dense dead cells are observed in the central retina (F, red) and
ath5-positive neurons markedly decrease in number (F, green), suggesting that
differentiating neurons undergo apoptosis in the piy mutant. Cells in
the CMZ incorporate BrdU (C,E, asterisks), suggesting that retinal stem cells
continue to proliferate in the piy mutant. (G,H) In
situ hybridization of wild-type (G) and piy mutant (H) retinas with
rx1 RNA probe (asterisks). (I,J) Plastic sections of
piy mutant retinas treated with DMSO (I) or TSA (J).
(K,L) TUNEL (green) of piy mutant retinas treated with
DMSO (K) or TSA (L). (M-P) Plastic sections of wild-type (M),
smu (N), piy (O) and smu; piy (P) mutant retinas.
Although retinal neurogenesis is delayed in the smu mutant, retinal
lamination occurs until 3 dpf (N). In the smu; piy double mutant,
retinal cell apoptosis is suppressed (P).
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