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Figure 5


Fig. 5. The β-TrCP orthologue lin-23 is differentially expressed during embryogenesis. (A) The progeny of transgenic hermaphrodites expressing GFP::LIN-23 exclusively in their germ line (pie-1 promoter) display ubiquitous and mainly cytoplasmic localization of maternal GFP::LIN-23 until the beginning of the E8-cell stage (~180 minutes). Asterisks indicate the intestinal cells. (B) Zygotic LIN-23::GFP is detected ~120 minutes after fertilization (E4-stage), and shows differential expression levels between the E lineage and the rest of the embryo. The differential expression of lin-23 continues throughout embryogenesis, with the highest levels in the head region and the lowest in the intestine. Intestinal cells were recognized by morphology, localization and the presence of birefringent gut granules (pseudo-red). The corresponding developmental time in minutes following pronuclear meeting is shown on the left.





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