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Fig. 3. Expression of Ascl1-GFP transgene in
PDGFR
+ OLPs. Images show transverse sections of
E16.5 spinal cords of Ascl1::GFP reporter mice. (A-D'')
Ascl1-GFP and PDGFR were stained in green and red, respectively, and
cell nuclei were stained with DAPI in blue. (E-H'') Co-staining
for Ascl1-GFP (green), PDGFR (blue) and endogenous Ascl1 protein (red).
Boxes indicate the locations of the areas shown in other panels. Broken lines
in B-B'' indicates the VZ. Arrowheads indicate the co-expression of GFP
and PDGFR, whereas arrows indicate
GFP-/PDGFR+ (C-C'') or
Ascl1-/GFP+/PDGFR+ cells
(F-F'',H-H''). Scale bars: 50 µm in A-A''; in
B'',C'',D'',E,G,F'' and H'', 20 µm for
B-B'',C-C'',D-D'',E,F-F'' and H-H''.
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