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Figure 7


Fig. 7. Defect in the co-expression of Olig2 and Nkx2-2 in Ascl1-/- spinal cords. (A) Co-expression of Olig2 and Nkx2-2 in vivo at E16.5. Arrowheads indicate Nkx2-2+/Olig2+ cells in the ventral white matter. Scale bar: 50 µm. (B,C) Expression of Nkx2-2 in Olig2+ OLPs in vitro. Spinal cord cells were isolated from either E16.5 (B) or E18.5 (C) embryos, and the expression of Nkx2-2 in Olig2+ cells was compared between the wild type and Ascl1-/- mutants at indicated time points. Data are mean±s.d. (n=3). *P<0.01 compared with DAP7; %P<0.01 compared with the wild type.





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