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Fig. 8. Promotion of oligodendrocyte development by Ascl1. Neurospheres
derived from E13.5 spinal cords were infected with GFP retroviruses expressing
various transcription factors either alone or in combination, and subsequently
induced to differentiate for 10 days. (A-D) Overexpression of Olig2,
Nkx2-2 and Ascl1 in virus-infected cells. Cells infected with control (A),
Olig2 (B), Nkx2-2 (C) and Ascl1 (D) viruses were stained for respective
transcription factors together with GFP (both in green; see lower panels) and
oligodendrocyte markers (red). Arrows indicate transgene expression in nuclei
of cells with GFP+ soma, whereas arrowheads indicate the expression
of endogenous factors in uninfected cells with GFP- soma.
(E,F) Differentiation of virus-infected cells into
NG2+ and GalC+ cells. The images show Ascl1
virus-infected cells expressing NG2 and GalC (arrowheads). Scale bars: in D
and F, 20 µm for A-D and E,F. (G,H) Percentages of
NG2+ and GalC+ cells among virus-infected cells
(mean±s.d. from three or four independent experiments). Parentheses
indicate the fold changes compared with the control level.
*P<0.01 compared with the control virus;
%P<0.01 compared with single infections.
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