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Figure 2


Fig. 2. Suppression of Lrig3 by a splicing morpholino disturbed embryonic development. (A) RT-PCR shows that injection of L3MO decreased the mature Lrig3 mRNA while inducing the predicted ~1 kb unspliced band, using intron-spanning primers for pseudoalleles Lrig3A and Lrig3B. ODC was loading control. (B-D) Phenotypes induced by L3MO. The morphants showed severe anterior defects, delayed or failed neural fold closure, and shortened axis. (C,D) Embryos were injected with 30 ng L3MO; uninjected control in B. (E-G) The phenotype was rescued by co-injection of Lrig3 mRNA. (E) Uninjected control; (F) L3MO (30 ng); (G) 30 ng of L3MO plus 20 pg Lrig3 mRNA.





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