spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Achieving PLK-1 asymmetry. (A-D) Wild-type (A), zen-4(RNAi) (B), tba-2(RNAi) (C) or nmy-2(RNAi) (D) embryos stained for PLK-1 (shown alone in the left panels and in red in the merged panels), {alpha}-tubulin (green) and DNA (blue). PLK-1 asymmetry is maintained despite cytokinesis failure (B). Whereas microtubules are not essential for PLK-1 asymmetry as the asymmetry is maintained and even slightly increased upon {alpha}-tubulin depletion (C), the actomyosin network is needed (D). (E,F) Wild-type (E) and partial plk-1(RNAi) (F) embryos stained for PLK-1 (shown alone in the left panels and in red in the merged panels), {alpha}-tubulin (green) and DNA (blue). Overall PLK-1 levels are diminished in partial plk-1(RNAi) embryos (F, top panel). To better illustrate the alteration in ratio between AB and P1, a second image of the same partial plk-1(RNAi) embryo is shown (F, bottom panel), in which intensities were increased so as to match those in AB in the wild-type embryo. (G) Average ratios, along with s.e.m., of PLK-1 in AB versus P1 in wild-type and partial plk-1(RNAi) embryos stained as in E and F. Wild type, 1.52±0.05, n=64; partial plk-1(RNAi), 1.97±0.13, n=16. Student's t-test, P=2.6x10-11. The star indicates that the difference with wild type is statistically significant.





Right arrow Return to article