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Fig. 6. Phenotypic analyses of double and triple mutants. (A) A
rev-9 seedling. (B) A severe ae5-1 rev-9 seedling
with only expanded cotyledons. An arrowhead indicates a needle-like leaf.
(C) A less severe ae5-1 rev-9 seedling showing rosette leaves
with rough leaf surface. (D) SEM of adaxial leaf surface of ae5-1
rev-9, showing that the abaxially featured epidermal cells appeared on
the adaxial leaf side (arrowhead). (E) An rdr6-3 seedling.
(F) An ae5-1 rdr6-3 seedling with lotus leaves (arrowheads).
(G,H) Transverse sections of rdr6-3 (G) and ae5-1
rdr6-3 (H) leaves. The positions of sectioning are indicated by white
lines in E and F. Arrowheads in G,H indicate the phloem. (I) SEM of a
leaf from ett-3 arf4-2 double mutant, showing a number of outgrowths
on the abaxial leaf side. (J) SEM of an ae5-1 ett-3 arf4-2
leaf, exhibiting a dramatically reduced number of the outgrowths. (K) A
kan1-2 kan2-1 seedling. (L) An ae5-1 kan1-2 kan2-1
seedling. Insets in K,L show the abaxial surface of the first-pair leaf;
arrowheads in J,K indicate the outgrowths. (M,N) Quantitative
analyses of outgrowths on the third-pair (M) and fourth-pair (N) rosette
leaves of ett-3 arf4-2 and ae5-1 ett-3 arf4-2. (O)
Quantitative analysis of outgrowths on the first-pair rosette leaves of
kan1-2 kan2-1 and ae5-1 kan1-2 kan2-1. Columns and error
bars in M-O represent the mean and s.d. Scale bars: 0.5 cm in A-C,E,F; 50
µm in D,G,H; 3 mm in I,J; 2 mm in K,L; 1 mm in K,L insets.
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