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Fig. 4. CPL3 gene expression. (A) Real-time PCR analysis of
CPL3 gene expression in Arabidopsis organs. Total RNA was
isolated from the indicated tissues. (B-E) In situ hybridization
patterns of CPC-homologous genes at the shoot apex. Arrowheads in B
and C indicate CPC and ETC1 signals evident in young
trichomes. No signal for either ETC2 or CPL3 was detected in
D and E. (F-M) Activity of CPL3p::GUS reporter in 2-week-old
leaves (F), in 5-day-old cotyledons (G), hypocotyls (H) and roots (I), in
4-week-old inflorescence (J,K) and silique (L,M). (N) Localization of
CPL3-GFP fusion protein. Fluorescence from the GFP fusion protein (green) and
propidium iodide (red) was observed with confocal laser scanning microscopy.
CPL3p::CPL3:GFP signal localization in 2-week-old leaves. Scale bars:
50 µm in B for B-E and in G,H,N; 100 µm in I; 200 µm in K,M; 1 mm in
F,J,L.
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