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Figure 1


Fig. 1. Analysis of STATc in slugs of a strain overexpressing PTP3 protein tyrosine phosphatase or its dominant-negative form. (A) PTP3 and slug morphology. The control slugs (left) are transformed with a myc-GFP overexpression construct, while those on the right overexpress PTP3. The arrows indicate points at which the slugs seem to have split apart. (B) PTP3 and STATc staining slugs derived from a non-transformed control and from cells transformed with myc:PTP3 or myc:PTP3CS, the dominant-negative form, were immunostained using a STATc antibody. In the control, STATc is nuclear enriched in the pstO region (double-headed arrow). In the slugs transformed with the PTP3 overexpressor, there is almost no detectable staining; in cells transformed with the dominant-negative PTP3, mutant PTP3 is nuclear enriched in all parts of the slug. (C) PTP3 and STATc activation level. Total cell lysate was prepared from slugs of parental Ax2 cells and PTP3OE cells. The samples were analysed using a tyrosine phosphorylation specific STATc antibody (CP22), total STATc antibody (7H3) and, as a loading control, a GSK-3 antibody.





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