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Fig. 2. Analysis of STATc activation by DIF-1 and osmotic stress in a PTP3
overexpressing strain. Parental Ax2 cells and PTP3OE cells were starved
for 4 hours and then left untreated, exposed to 100 nM DIF-1 (A) for 5
minutes or exposed to 200 mM sorbitol (B) for 5 minutes. One aliquot of
cells was lysed, subjected to western transfer and the blot was analysed with
an antibody specific to the tyrosine phosphorylated form of STATc. As a
loading control, a parallel blot was probed with total STATc antibody. The
second aliquot of cells was fixed and immunostained for STATc.
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