spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. FRAP analyses of {alpha}-catenin-GFP. (A) Dorsal branch of btl>{alpha}-catenin-GFP embryos with or without Src42AACT were photo bleached at the bracketed regions and fluorescence recovery was recorded. (B) Schematic plot of FRAP analysis. Photo bleaching reduces the fluorescence level from pre bleach (Fpre) to post bleach (Fpost). Fast recovery of fluorescence reaches an approximate plateau level (Fend) that is usually lower than Fpre. Recovery to Fpre required a longer time and was not analyzed. Mobile fraction [Mf=100x(Fend-Fpost)/(Fpre-Fpost)] and half-time of recovery (t1/2) were estimated from the plots. (C) Representative plots of fluorescence recovery in dorsal branches of control (black), Src42A; Src64B double mutant (red) and Src42AACT-expressing (blue) embryos. (D) t1/2 and Mf values under various conditions of Src activities. Error bar indicates standard error. Values that deviate from controls are indicated (*P<0.05, **P<0.005).





Right arrow Return to article