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Fig. 2. Wnt5a mis/overexpressed lungs show pulmonary hypoplasia with
abnormal air and vascular tubulogenesis. (A-C) Whole lungs
dissected from wild-type (WT, A), RCAS-Wnt5a-infected (B), and
RCAS-Wnt3a-infected (C) embryos at E14, showing pulmonary hypoplasia
of the infected lungs. (D-I) H&E-stained lungs showing dramatic
thinning of the airway epithelium by mis/overexpression of Wnt5a
(compare brackets in E with D) but not following RCAS-Wnt3a infection
(compare brackets in G with F). RCAS-Wnt3a mis/overexpressed lungs at
E15 have more normal appearing airways and airway epithelial thickness
(compare G with F) but develop with abnormal interstitial muscularized
vessels, often with extravasated red blood cells (arrows and insert, G). At
E20, Wnt5a mis/overexpressing lungs often show pulmonary
lymphangiectasia (arrows in I) and disordered larger interstitial vessels
(arrowheads, I; compare with wild-type lungs, H). (J-L) At E14, normal
developing pulmonary endothelium is localized between airways or parabronchi,
as Elderberry bark lectin shows (red arrow, J). With RCAS-Wnt5a
infection, the developing vasculature clusters subepithelial to the
parabronchi (red arrow, K). The number of elderberry bark lectin-stained
interstitial vessels are increased by Wnt3a mis/overexpression but
these vessels are localized normally in the interstitium (L), not clustered
subepithelially as seen with Wnt5a mis/overexpression (K). Nuclei
were counterstained with DAPI (blue). (M,N,O) Cartoon
tracings of J,K,L make pattern changes of vasculature clearer. Epithelial
cells are orange; endothelial cells, green.
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