spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Wnt5a affects the expression of adhesion factors. Lungs imaged in A-G are all from in-ovo experiments; H-J are from explant (Exp) experiments. (A) L-CAM antibody localizes in the epithelium in wild-type lungs at E14 (red arrowhead, inset); arrow indicates the distal airway magnified in inset. (B) In Wnt5a-mis/overexpressing lungs, L-CAM expression is observed in the proximal epithelium (red arrow), but is reduced in the most-distal budding epithelium (red arrowhead, inset). (C) At E14 in wild-type lungs, fibronectin is principally expressed subepithelially (arrow, inset) and in the interstitial vasculature (red arrowhead, inset). (D) At this stage, fibronectin levels are increased diffusely in RCAS-Wnt5a-injected lungs as compared with wild type. (E,F) The fibronectin levels remain altered through to E20, when fibronectin is normally localized around the parabronchi lumen, surrounding the atrial muscle (red arrow, inset, E), in few distal luminal epithelial cells, and in the interstitial vasculature (red arrowhead, E). With Wnt5a mis/overexpression (F), fibronectin is overexpressed diffusely (red arrow shows a similar pattern surrounding the atrial muscle as in E, but note the diffuse expression throughout epithelium); however, almost no expression of fibronectin can be detected clustered in the interstitium between parabronchi (red arrowhead). (G) At E14, Wnt3a mis/overexpression does not significantly affect the level or pattern of fibronectin expression (compare with wild type in C). (H) In wild-type explants, normal fibronectin expression is observed subepithelially (red arrow) and in the interstitial vasculature (red arrowhead). (I) dnRor2 mis/overexpression decreases fibronectin expression overall and in the subepithelium (red arrow) and the interstitial vessels (red arrowhead). (J) Explants incubated with Dkk1 recombinant protein fail to develop vessels but show near normal subepithelial fibronectin expression (red arrow, J; compare with H).





Right arrow Return to article