|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Normal cell death and cell proliferation in the distal mesenchyme of
Fgfr2AER-KO forelimb buds. (A-D) Cell death as
detected by TUNEL assay (green) at the stages indicated. Note that there were
more dying cells in the AER and ventral ectoderm of mutant forelimb buds than
in controls at the 30 s (B; n=6) and the 36 s (D; n=6)
stages. (E-L) Cell proliferation as detected via BrdU incorporation
(E-H',M) and pH3 immunofluorescence (I-L,N), which marks nuclei in cells
undergoing mitosis, in the forelimb buds of mutant and control embryos. White
circles indicate the areas (
=200 µm) of sub-AER mesenchyme in which
BrdU-(M) or pH3-(N) positive cells were quantified. Values are
the mean±s.d. for each data point in M and the mean±s.e.m. for
each data point in N. No statistically significant differences in the
percentage of BrdU-positive cells or in the number of pH3-positive cells were
observed between the control and mutant limb buds at the stages indicated
(unpaired, two-tailed Student's t-test). E11.5 is equivalent to
45 s stage. Scale bars: 50 µm. Abbreviations: an, anterior; di,
distal; do, dorsal; pr, proximal; po, posterior; ve, ventral; s, somite.
|
